Figure 1.
Validation of unique trauma + trigger phenotype. A, Timeline of shock exposure as part of trauma plus trigger and control protocols, behavioral tests, and collection of biological samples. B, Trauma plus trigger mice show significantly less percentage of time in the light/dark paradigm engaged in risk assessment behavior (F(3) = 30.7257, p < 0.0001, n = (9–50); planned contrasts F(89) = 68.9777, p < 0.000000000001). C, The significantly fastest startle response upon exposure to startle stimulus (F(3) = 5.6378, p < 0.0015, n = 16–30; planned contrast F(80) = 15.42775, p < 0.00018). D, Significantly more activity in the nonactive phase of the light/dark cycle (F(3) = 5.6378, p < 0.0001, n = 10–15; planned contrasts F(52) = 31.7737, p < 0.0000007). E, Sample activity in both phases of the light cycle in which trauma plus trigger exposed mice are the most active (***p < 0.0001).