Figure 2.

ERG analysis of HR2.1:TN-XL mice versus wt mice. A, Representative photopic single-flash ERGs in 1-month-old HR2.1:TN-XL (red) and wt (black) mice under a static background light (30 cd/m²). The arrow indicates the b-wave. B, Box-and-whisker plot showing photopic single-flash b-wave amplitudes in wt (black) and HR2.1:TN-XL (red) mice. Boxes indicate the 25% and 75% quantile range, whiskers indicate the 5% and 95% quantiles, and the asterisks indicate the median of the data. The amplitude differences were not statistically significant at any intensity (p = 0.13 to 0.67 for −0.5 to 1.5 cd · s/m²). C, Overlay of the response traces of wt (black) and HR2.1:TN-XL (red) mice from A. D, Representative flicker ERG response traces at 18 and 20 Hz under dark-adapted condition in wt (black) and HR2.1:TN-XL (red) mice. E, Box-and-whisker plot showing flicker response amplitudes of wt (black) and HR2.1:TN-XL (red) mice (p = 0.31 for 18 Hz, p = 0.39 for 20 Hz). F, Representative scotopic single flash ERGs in wt (black) and HR2.1:TN-XL (red) mice. Arrows indicate the a- and b-waves. G, Box-and-whisker plot of scotopic single-flash a- and b-wave amplitudes in wt (black) and HR2.1:TN-XL (red) mice. The amplitude differences were not statistically significant at any intensity (p = 0.093–1.00 for −1.5–1.5 cd · s/m² for the a-wave, p = 0.39–0.94 for −4–1.5 cd · s/m² for the b-wave). H, Overlay of the response traces of wt (black) and HR2.1:TN-XL (red) mice from F.