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. 2012 Jul 4;32(27):9383–9395. doi: 10.1523/JNEUROSCI.6363-11.2012

Figure 1.

Figure 1.

The expression of Trks and TRPCs in rat hippocampus. A, Western blots of total lysates extracted from rat hippocampus at different developmental stages with indicated antibodies. α-Tubulin served as a loading control. B, Representative staining of cultured hippocampal neurons at 7 DIV with the indicated antibodies or dyes. Right, Ninefold magnification of the white rectangle areas in “Merge.” Bottom, Representative staining of culture astrocytes at 7 DIV labeled with Mitotracker anti-HSP60 for mitochondria and Hoechst33342 for nucleus. Scale bar: 20 μm. C, D, Quantification of the colocalization of TRPCs and Trks shown in B. Lower limit: HSP60 and Hoechst33342, PCC, 0.202 ± 0.028; MOC, 0.042 ± 0.008. Upper limit: HSP60 and Mitotracker, PCC, 0.903 ± 0.020; MOC, 0.931 ± 0.032. In each group, 4–11 pictures were analyzed, *p < 0.05; **p < 0.01.