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. 2012 Jul 4;32(27):9383–9395. doi: 10.1523/JNEUROSCI.6363-11.2012

Figure 6.

Figure 6.

NT-3 or NT-4 regulated dendritic growth via TRPC5 or TRPC6, respectively. A, Representative images for the neurons transfected with shTRPC5i or shTRPC6i and stimulated with NT-3. B, Quantification of total dendritic tips for the neurons shown in A. Scramble vehicle: 27.50 ± 1.35; scramble NT-3: 21.73 ± 1.22; shTRPC5i vehicle: 33.21 ± 1.93; shTRPC5i NT-3: 29.59 ± 1.67; shTRPC6i vehicle: 22.07 ± 1.76; shTRPC6i NT-3: 18.00 ± 2.48. C, Quantification of total dendritic length for the neurons shown in A. In μm, scramble vehicle: 1231.00 ± 64.36; scramble NT-3: 785.90 ± 50.85; shTRPC5i vehicle: 1529.00 ± 86.70; shTRPC5i NT-3: 1504.00 ± 85.68; shTRPC6i vehicle: 818.08 ± 43.00; shTRPC6i NT-3: 595.70 ± 76.82. *p < 0.05; **p < 0.01. D, E, Sholl analyses of the neurons shown in A. F, Representative images for the neurons transfected with shTRPC6i and stimulated with NT-4. G, Quantification of total dendritic tips for the neurons shown in F. Scramble vehicle: 27.50 ± 1.93; scramble NT-4: 40.30 ± 3.54; shTRPC6i vehicle: 17.02 ± 1.00; shTRPC6i NT-4: 20.60 ± 1.41. H, Quantification of total dendritic length for the neurons shown in F. In μm, scramble vehicle: 1231.43 ± 97.46; scramble NT-4: 1735.08 ± 85.61; shTRPC6i vehicle: 722.05 ± 35.50; shTRPC6i NT-4: 746.78 ± 38.08. *p < 0.05; **p < 0.01. I, J, Sholl analyses of the neurons shown in F. Culture hippocampal neurons were transfected with indicated shRNAi at 3 DIV, stimulated with neurotrophin at 5 DIV for 48 h and observed at 7 DIV. Scale bar: 50 μm. Data were means ± SEM from three independent experiments with >30 neurons in each group. The numbers of neurons analyzed are shown inside the bars.