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Preembedding (A, B) and postembedding (C–H) immunogold electron microscopy showing loose and incomplete assembly of presynaptic molecules for GABAergic synaptic transmission around the AIS of PCs. A–D, GAD. E, F, VIAAT. G, H, Bassoon (Bass). BC synapses on PC somata (So) are shown in A, C, E, and G (filled arrowheads), whereas BC axons contacting the AIS of PCs are in B, D, F, and H (open arrowheads). Asterisks indicate BC axons and terminals. Scale bars, 250 nm.
