Table 1.
Raw RI of all genotypes in different experimental conditions
| Genotype | Exposure | Pretest response (n) [to odor] | Posttest response (n) [to odor] |
|---|---|---|---|
| CS | 5% EB | 0.73 ± 0.02 (13) [10−3 3 Oct] | 0.68 ± 0.02 (13) [10−3 3 Oct] |
| 5% EB | 0.51 ± 0.01 (9) [10−2 3 Oct] | 0.50 ± 0.01 (9) [10−2 3 Oct] | |
| 5% EB | 0.71 ± 0.02 (18) [10−3 EB] | 0.38 ± 0.01 (18) [10−3 EB] | |
| ShakB2 | 5% EB | 0.66 ± 0.02 (9) [10−3 EB] | 0.50 ± 0.03 (9) [10−3 EB] |
| CS > Shi ts1 | 5% EB at 25°C | 0.66 ± 0.02(8) [10−3 EB] | 0.34 ± 0.02 (8) [10−3 EB] |
| 5% EB at 32°C | 0.39 ± 0.02 (8) [10−3 EB] | ||
| GH146 > Shi ts1 | 5% EB at 25°C | 0.65 ± 0.01 (9) [10−3 EB] | 0.30 ± 0.02 (5) [10−3 EB] |
| 5% EB at 32°C | 0.56 ± 0.02 (9) [10−3 EB] | ||
| CS > Shi ts1 | 5% EB at 25°C | 0.68 ± 0.02 (4) [10−3 EB] | 0.30 ± 0.02 (8) [10−3 EB] |
| 5% EB at 32°C | 0.34 ± 0.02 (8) [10−3 EB] | ||
| MB247 > Shi ts1 | 5% EB at 25°C | 0.69± 0.02 (7) [10−3 EB] | 0.32 ± 0.04 (6) [10−3 EB] |
| 5% EB at 32°C | 0.39 ± 0.03 (10) [10−3 EB] | ||
| GH146 > TRPA1 | 29°C | 0.72 ± 0.03 (10) [10−3 EB] | 0.33 ± 0.04 (10) [10−3 EB] |
| VPN > TRPA1 | 29°C | 0.65 ± 0.03 (11) [10−3 EB] | 0.57 ± 0.02 (11) [10−3 EB] |
| GH146 > TRPA1/dsNR1RNAi | 29°C | 0.74 ± 0.03 (8) [10−3 EB] | 0.65 ± 0.05 (9) [10−3 EB] |
| GH146 > TRPA1/RdlRNAi | 29°C | 0.64 ± 0.01 (9) [10−3 EB] | 0.54 ± 0.02 (10) [10−3 EB] |
| GH146,LN1 > TRPAp1 | 29°C | 0.68 ± 0.02 (10) [10−3 EB] | 0.37 ± 0.02 (7) [10−3 EB] |
| GH146,LN1 > VGLUT i;TRPA1 | 29°C | 0.69 ± 0.02 (9) [10−3 EB] | 0.60 ± 0.02 (10) [10−3 EB] |
| GH146,LN1 > RutRNAi;TRPA1 | 29°C | 0.67 ± 0.01 (10) [10−3 EB] | 0.56 ± 0.01 (11) [10−3 EB] |
| ShakB2;GH146 > TRPA1 | 29°C | 0.65 ± 0.04 (9) [10−3 EB] | 0.58 ± 0.03 (9) [10−3 EB] |
| VPN > TRPA1 | 29°C | 0.70 ± 0.03 (8) [5% CO2] | 0.30 ± 0.02 (9) [5% CO2] |
| GH146 > TRPA1 | 29°C | 0.68 ± 0.03 (9) [5% CO2] | 0.61 ± 0.05 (10) [5% CO2] |
| VPN > TRPA1;dsNR1RNAi | 29°C | 0.64 ± 0.02 (10) [5% CO2] | 0.57 ± 0.02 (10) [5% CO2] |
| VPN > TRPA1;RdlRNAi | 29°C | 0.70 ± 0.02 (11) [5% CO2] | 0.60 ± 0.04 (12) [5% CO2] |
| +/TRPA1 | 5% EB | 0.58 ± 0.02 (9) [10−3 EB] | 0.26 ± 0.01 (8) [10−3 EB] |
| 29°C | 0.60 ± 0.03 (8) [10−3 EB] | 0.56 ± 0.03 (9) [10−3 EB] | |
| 5% EB + 29°C | 0.64 ± 0.04 (5) [10−3 EB] | 0.30 ± 0.03 (9) [10−3 EB] | |
| GH146 > TRPA1 | 5% EB | 0.60 ± 0.02 (8) [10−3 EB] | 0.26 ± 0.02 (7) [10−3 EB] |
| 29°C | 0.57 ± 0.03 (9) [10−3 EB] | 0.30 ± 0.03 (9) [10−3 EB] | |
| 5% EB + 29°C | 0.61 ± 0.04 (10) [10−3 EB] | 0.23 ± 0.03 (10) [10−3 EB] | |
| +/TRPA1 | 15% CO2 | 0.70 ± 0.02 (6) [5% CO2] | 0.36 ± 0.01 (6) [5% CO2] |
| 29°C | 0.64 ± 0.01 (8) [5% CO2] | 0.61 ± 0.02 (7) [5% CO2] | |
| 15% CO2 + 29°C | 0.62 ± 0.02 (8) [5% CO2] | 0.28 ± 0.03 (8) [5% CO2] | |
| VPN > TRPA1 | 15% CO2 | 0.67 ± 0.02 (6) [5% CO2] | 0.40 ± 0.01 (8) [5% CO2] |
| 29°C | 0.58 ± 0.01 (8) [5% CO2] | 0.36 ± 0.01 (9) [5% CO2] | |
| 15 %CO2 + 29°C | 0.61 ± 0.02 (10) [5% CO2] | 0.36 ± 0.03 (11) [5% CO2] | |
| GH146 > TRPA1 | 29°C | 0.63 ± 0.03 (10) [10−3 EB] | 0.30 ± 0.02 (9) [10−3 EB], 0 h |
| 0.48 ± 0.02 (9) [10−3 EB], 1 h | |||
| 0.56 ± 0.03 (9) [10−3 EB], 3.5 h | |||
| VPN > TRPA1 | 29°C | 0.59 ± 0.02 (9) [5% CO2] | 0.19 ± 0.01 (8) [5% CO2], 0 h |
| 0.36 ± 0.02 (8) [5% CO2], 1 h | |||
| 0.52 ± 0.03 (8) [5% CO2], 3.5 h | |||
| GH146 > TRPA1 | 29°C | 0.60 ± 0.02 (5) [10−3 EB] | 0.24 ± 0.03 (7) [10−3 EB] |
| 29°C + vortexing | 0.55 ± 0.02 (8) [10−3 EB] | ||
| VPN > TRPA1 | 29°C | 0.63 ± 0.04 (7) [5% CO2] | 0.29 ± 0.02 (8) [5% CO2] |
| 29°C + vortexing | 0.56 ± 0.03 (8) [5% CO2] | ||
| GH146 > TRPA1 | 29°C | 0.70 ± 0.04 (6) [10−3 EB] | 0.40 ± 0.03 (6) [10−3 EB] |
| 29°C + yeast puff | 0.59 ± 0.03 (6) [10−3 EB] | ||
| VPN > TRPA1 | 29°C | 0.62 ± 0.02 (6) [5% CO2] | 0.28 ± 0.01 (6) [5% CO2] |
| 29°C + EB pulse | 0.56 ± 0.03 (6) [5% CO2] |
n is the number of sets done for each condition; values are ± SEM. 3 Oct, 3-Octanol.