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. 2012 Jun 27;32(26):9053–9058. doi: 10.1523/JNEUROSCI.0260-12.2012

Figure 1.

Figure 1.

Cellular effects of bone marrow cell transplantation in the olfactory bulb of PCD mice. A–C, GFP+ BMDCs (green) expressed microglial marker (Iba1 in red) in the olfactory bulb (counterstaining with DAPI, blue) and display a highly ramified morphology typical of microglia. Scale bar, 200 μm. D, E, MC somata (arrows) in PCD (D) and grafted PCD mice (E) labeled with antibodies against reelin (red). GL, Glomerular layer; EPL, external plexiform layer; MCL, mitral cell layer; GCL, granule cell layer. Scale bars, 100 μm. F, Evolution of MC density in PCD and grafted PCD animals. Student's t test, **p < 0.01. G–I, Coronal view of a transplanted mouse OB. GFP+ BMDCs (green; G, I) were spread throughout the entire olfactory bulb, displayed microglial morphology (G, I, inset), but never colocalized with reelin (red; H, I). Scale bar, 250 μm; inset, 100 μm. J, K, Proliferative Ki67-positive cells in the subventricular zone in PCD (J) and grafted PCD [K, counterstaining with propidium iodide (PI)]. LV, Lateral ventricle. Scale bars, 75 μm. L, Quantification of the proliferation rate (percentage of Ki67+ cells) in the SVZ and RMS in WT (white), grafted WT (green), PCD (hatched white), and grafted PCD (hatched green). Student's t test, **p < 0.01.