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. 2019 Jun 21;30(7):1238–1249. doi: 10.1681/ASN.2018111089

Figure 1.

Figure 1.

Human light chain Cκ domain (hLC) substitution does not affect IgA deposition in hα1+/+ knock-in κ-switch (KS+/+) mice. (A) Plasma hIgA levels in hα1+/+ and hα1+/+KS+/+ mice at 6, 12, 24, and 48 weeks in both a specific and opportunistic pathogen-free facility (SOPFF) and a conventional facility (CF). Results are the means ± SEM of n=6–10 mice per group. *P<0.05; **P<0.01. (B) Analysis of monomeric IgA (mIgA) and polymeric IgA (pIgA) by western blot at 48 weeks; molecular mass scale is expressed in kilodaltons. Blots are representative of n=6 mice per group, and two mice from each group are presented. (C) hIgA and mC3 deposits in mice kidneys at 48 weeks in CF (podocin in red, hIgA in green, and mC3 in yellow). (D) Hematoxylin and eosin staining of kidney sections from mice at 48 weeks. All pictures are representative for glomeruli found in kidney sections of n=6–12 mice per group. hIgA, human IgA.