Identification of tissue-resident MAIT cells in healthy human kidney tissue. (A) Gating strategy used to identify MAIT cells (CD3+ TCR Vα7.2+ CD161hi mononuclear cells) in healthy human kidney tissue. Representative flow cytometric data from one of 11 individual donors are shown. (B) Relative expression of TCR Vα7.2, CD161, CD8, IL-18Rα, IL-7Rα, CCR5, CXCR3, CD103, and CD69 by MAIT cells (black unfilled) and total T cells (gray filled) compared with isotype (dashed). Representative data from four to 11 individual donors are shown. FSC-A, forward scatter-area; SSC-A, side scatter-area.