Figure 7.

MAIT cells activated in the presence of hypoxic PTECs produce significantly increased levels of cytotoxic molecules. (A and C) (A) Perforin and (C) granzyme B production by MAIT cells after 24-hour culture without (−) or with (+) preconditioned PTECs under normoxic or hypoxic conditions in the absence (24-hour) or presence (24-hour IL) of IL-12p70, IL-15, and IL-18. One representative donor experiment is shown. (B and D) Fold changes (concentration in the presence of PTECs/concentration in the absence of PTECs; +/− PTECs) in cytotoxic molecule production by IL-stimulated MAIT cells under normoxic and hypoxic conditions for seven individual donor PTEC experiments. Symbols represent individual donor PTEC experiments; the representative donor experiment from (A and C) is identified using filled triangles. Horizontal bars represent medians, with interquartile range also presented. *P<0.05, Wilcoxon matched-pairs signed-rank test.