Figure 5.

GTP-hydrolyzing activity of Era. (A) GTPase activity of Era in the absence of RNA, in the presence of the ₁₅₃₁AUCACCUCCUUA₁₅₄₂ sequence, in the presence of the sequence with 1531–1534 mutations, and in the presence of the sequence with 1535–1539 mutations. (B) GTPase activity of Era in the presence of the ₁₅₃₁AUCACCUCCUUA₁₅₄₂ sequence with a single mutation at each position from 1531 to 1539 or the deletion of nucleotide 1531. (C) GTPase activity of Era in the presence of the ₁₅₃₀GAUCACCUCCUUA₁₅₄₂ sequence, in the presence of the sequence with a single or double mutation at 1530 and/or 1531, or with the deletion of nucleotide 1530 and a single mutation at 1531. (D) GTPase activity of Era in the presence of longer RNA consisted of h45 (nucleotides 1506–1529) and the ₁₅₃₀GAUCACCUCCUUA₁₅₄₂ sequence, either native (RNA301) or with a G1530A mutation. Data were taken from References [12, 13] and presented graphically here.