KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-EpCam-APC-Cy7 (Clone G8.8) | Biolegend | Cat# 118218 |
| Anti-CD45-PacBlue (Clone 30-F11) | Biolegend | Cat# 103126 |
| Anti-NS1/2 (Rabbit polyclonal) | provided by Vernon Ward | N/A |
| Anti-NS6/7 (Guinea-pig polyclonal) | provided by Kim Green | N/A |
| Anti-NS1 (CM79, mouse monoclonal) | provided by Vernon Ward | N/A |
| Anti-capsid (A6.2, mouse monoclonal) | This study | N/A |
| Anti-cleaved Casp3 (D175)-AF647 (Clone C92-605) | BD | Cat# 560626 |
| Bacterial and Virus Strains | ||
| MNoV-CR6 | (Strong et al., 2012) | N/A |
| MNoV-CR6D121G | This study | N/A |
| MNoV-CR6D131G | This study | N/A |
| MNoV-CR6D121/131G | This study | N/A |
| MNoV-CW3 | (Strong et al., 2012) | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant IFN-λ | Bristol-Myers Squibb | N/A |
| Recombinant NS1 | This study | N/A |
| Recombinant VP1P-domain | This study | N/A |
| C12R | PBL | Cat# 12185-1 |
| Critical Commercial Assays | ||
| ZR-96 viral RNA kit | Zymo Research | Cat# R1041 |
| Direct-zol-96 RNA kit | Zymo Research | Cat# R2056 |
| ImPromll reverse transcriptase | Promega | Cat# PRA3803 |
| Luciferase Assay System | Promega | Cat# E1500 |
| PrimeTime qPCR assay (Ifi35) | IDT | Cat# Mm.PT.58.42591180 |
| PrimeTime qPCR assay (Ifit1) | IDT | Cat# Mm.PT.58.32674307 |
| PrimeTime qPCR assay (Oas1a) | IDT | Cat# Mm.PT.58.30459792 |
| Experimental Models: Cell Lines | ||
| BV2 | N/A | |
| HEK293T | N/A | |
| Mx2LUC-IEC | Inscreenex | Cat# INS-CI-1007L |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | Jackson Laboratory | Stock # 000664 |
| Mouse: Ifnar1−/−(B6.129.Ifnar1tm1) | (Muller et al., 1994) | N/A |
| Mouse: Ifnlr1−/− (B6.Cg-Ifnlr1tm1Palu) | (Ank et al., 2008; Baldridge et al., 2017) | N/A |
| Mouse: Ifngr1−/− (JAX B6.129S7-Ifngr1tm1Agt/J, | Jackson Laboratory | Stock # 003288 |
| Mouse: Casp1/11−/− (JAX B6N.129S2-Casp1tm1Flv/J) | Jackson Laboratory | Stock # 016621 |
| Mouse: Rag1−/− (B6.129S7-Rag1tm1Mom/J) | (Mombaerts et al., 1992) | N/A |
| Mouse: Ifnlr1f/f (Ifnlr1tm1a(EUCOMM)Wtsl) | (Baldridge et al., 2017) | N/A |
| Mouse: Villin-Cre | (Madison et al., 2002) | N/A |
| Oligonucleotides | ||
| MNoV qPCR forward: 5′-CACGCCACCGATCTGTTCTG-3′ | IDT | N/A |
| MNoV qPCR reverse: 5′-GCGCTGCGCCATCACTC-3′ | IDT | N/A |
| MNoV qPCR probe: 5′-6FAM-CGCTTTGGAACAATG-MGBNFQ-3′ | Thermo Fisher Scientific | N/A |
| Rps29 qPCR forward: 5′-CAAATACGGGCTGAACATG-3′ | IDT | N/A |
| Rps29 qPCR reverse: 5′-GTCCAACTTAATGAAGCCTATGTC-3′ | IDT | N/A |
| Rps29 qPCR probe: 5′-HEX-CCTTCGCGTACTGCCGGAAGC-3′ | IDT | N/A |
| Software and Algorithms | ||
| Prism 7 software (V7.02) | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| FACSDiva software (V8.0.1) | BD | http://www.bdbiosciences.com/us/instruments/research/software/flow-cytometry-acquisition/bd-facsdiva-software/m/111112/overview |
| FlowJo (V10.1) | FlowJo | https://www.flowio.com/solutions/flowjo |