Fig 8. COA-C significantly induced PDE activity in hiPSC-CMs.

PDE enzyme (20 mU/well) was incubated at 30°C for the indicated times with cAMP (200 μM) and 5’-nucleotidase (50 kU/well) with or without COA-Cl (1 mM). Reactions were terminated by the addition of 100 μL of Green Assay Reagent and OD 620 nm read 30 min later. A standard curve of cAMP can be used to convert the OD 620 nm data to nmol of 5’-AMP. Each data point represents the mean and SEM of three independent experiments performed in triplicate.