Fig 11. Egr-Grnd is not essential for endogenous JNK signaling activation in cyst cells.

(A-D) Testes from unmated 1w-old puc-lacZ^B48 males immunostained for β-Galactosidase, co-stained for FasIII to mark hubs (asterisks). LacZ expression was comparable between heterozygous controls and the homozygous mutants for egr¹ (A and B) and grnd^minos (C and D). (E) Numbers of Zfh-1-positive cells in the testes from unmated 5- and 31-day-old males. Numbers of testes scored are shown at the bottom of each column. Statistic significances were calculated with student t test in E, and ns: p>0.05, *p<0.05, ***p<0.001. Unmated males (30 vs. 0) were examined in all experiments.