Table 4. PM-induced cytotoxicity and M.tb-induced host immune responses in seasonal PM-pre-exposed PBMC.
| Increased Host Response | R2.5 | CD2.5 | WD2.5 | R10 | CD10 | WD10 |
|---|---|---|---|---|---|---|
| PM-induced Cytotoxicity (LDH, Fig 1A–1D) |
n.s. | n.s. | n.s. | n.s. | + | + |
| Phagocytosis (% M.tb uptake, Fig 2) |
- | - | n.s. | - | - | - |
| PM-induced IFN-γ (spot frequency (Fig 3A–3D) |
+ | + | + | + | + | + |
| PM-induced IL-1β (spot frequency (Fig 3E–3H) |
+ | + | n.s. | + | n.s. | n.s. |
| PM + M.tb-induced IFN-γ in IGRA-/IGRA+ subjects (spot frequency, Fig 4A–4D) |
-/- | n.s./- | n.s./- | n.s./- | -/- | -/- |
| PM + PPD-induced IFN-γ in IGRA-/IGRA+ subjects (spot frequency, Fig 4E–4H) |
n.s./- | n.s./- | n.s./- | n.s./- | - | - |
| PM + M.tb-induced IL-1β in IGRA-/IGRA+ subjects (spot frequency, Fig 6A–6D) |
n.s./- | n.s./n.s. | +/- | n.s./n.s. | -/- | -/- |
| Loss of M.tb growth control (CFU on day 4, 5μg/ml, Fig 7) |
n.s./+ | +/+ | n.s./+ | n.s./+ | +/+ | +/+ |
n.s. indicates no statistical significance relative to control unexposed PBMC; + or–indicates statistically significant increase (+) or decrease (-) relative to control PBMC; / indicates comparison between IGRA- and IGRA+ subjects.