Fig 1. SOCE is required in Crz neurons for larval development on NR media.

(A) Expression pattern of Crz-GAL4 driver, used in this study to manipulate Crz neurons, visualised by expressing membrane bound RFP (mRFP) and GFP with a nuclear localisation signal (GFP^nls) (B) % Pupae upon reduction of SOCE by knockdown of STIM (STIM^IR), IP₃R (IP3R^IR) or ectopic expression of a dominant-negative Orai (Orai^E180A) in Crz neurons. To measure pupariation, twenty five, 88h±3h old larvae, per vial, were transferred to either normal food (corn flour, yeast, sugar) (See materials and methods for exact composition) or nutrient restricted (NR; 100mM Sucrose) media and number of pupae (and adults where relevant) that developed were counted. N = 6 vials for all experiments in this study. (C) % Pupae upon reduction of either sNPF or Crz (Crz^IR, sNPF^IR) in Crz neurons, and when, dSTIM or IP₃R are expressed in this background (dSTIM O/E; IP₃R O/E). (D) % Adults recovered for genotypes in (C). Bars with the same alphabet represent statistically indistinguishable groups. Two-way ANOVA with Sidak multi comparison p<0.05 for (B), (C) and (D). See also S1 Fig and for source data, S1 Table.