Fig 2. Crz⁺ and sNPF⁺ DLPs are required for development on NR media and activated by NR.

(A) Cartoon of Crz⁺ and sNPF⁺ neurons in the larval CNS marked by Crz-GAL4. DLP: dorso lateral peptidergic; VG: Ventral Ganglion; SEZ: Subesophageal zone (B) % Pupae when dSTIM is selectively down-regulated only in Crz⁺ and sNPF⁺ DLPs, by using the tsh-Gal80 transgene and in the presence of dicer2. Control: tshGal80/+;dSTIM^IR/+. Data represents mean ± SEM (C) Representative image. Expression of the UV-activated Ca²⁺ indicator, CaMPARI in Crz⁺ and sNPF⁺ DLPs, in larvae on 24 hours of normal (Fed) or NR media (24h NR). Fluorescence at 561nm (F₅₆₁) reflects Ca²⁺ levels, while at 488nm (F₄₈₈) reflects levels of the indicator CaMPARI. (D) Quantification of Ca²⁺ levels as reported by F_561/488 ratio in DLPs in larvae on 24 hours of normal or NR media, in the presence and absence of UV-stimulation. N>7 larvae for UV-stimulated; N = 3 for No UV stimulation. Bars with the same alphabet represent statistically indistinguishable groups. Two-way ANOVA with Sidak multi comparison test p<0.05 for (B). Mann-Whitney Test for (D). See also S2 Fig and for source data, S2 Table.