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. 2019 Jul 11;14(7):e0219715. doi: 10.1371/journal.pone.0219715

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© 2019 Dueñas-Santero et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — (A) Growth in selective media. Single colonies of strains SEP7-HA::LAL/SEP7 (2), SEP7-HA::LAL/SEP7 CDC10-HA::LHL/CDC10 (3), SEP7-HA::LAL/SEP7 CDC10-HA::LHL/CDC10 CDC12-HA::NAT1-Clox/CDC12 (4) and SEP7-HA::loxP/SEP7 CDC10-HA::loxP/CDC10 CDC12-HA::lox/CDC12 (5) were grown on YPD plates and replica-printed to SC -Arg, SC -His or YPD+ nourseothricin media. (B) Western blot analysis of the same strains using anti-HA antibody. The BWP17 parental strain (1) was included as an untagged control. Protein extracts were separated on 4–12% Bis-Tris gels, blotted to PDVF membranes and incubated with anti-HA antibody.