Table 1. The pFA-Clox toolkit.
Plasmids generated and fragment length of the cassettes.
| Plasmid | Accession number | Selectable Marker | Gene | 5´ end | 3´ end | Cassette Size |
|---|---|---|---|---|---|---|
| pFA-LAL | MK652110 | loxP-ARG4-loxP | - | S1 | S2 | 2180 |
| pFA-LHL | MK652111 | loxP-HIS1-loxP | - | S1 | S2 | 1474 |
| pFA-LUL | MK652112 | loxP-URA3-loxP | - | S1 | S2 | 1580 |
| pFA-NAT1-Clox | MK652113 | loxP-NAT1-cre-loxP | - | S1 | S2 | 4576 |
| pFA-URA3-Clox | MK652114 | loxP-URA3-cre-loxP | - | S1 | S2 | 4344 |
| pFA-GFPγ-LAL | MK652105 | loxP-ARG4-loxP | GFPγ | S1-XFP | S2 | 3142 |
| pFA-GFPγ-LHL | MK652106 | loxP-HIS1-loxP | GFPγ | S1-XFP | S2 | 2436 |
| pFA-GFPγ-LUL | MK652107 | loxP-URA3-loxP | GFPγ | S1-XFP | S2 | 2542 |
| pFA-GFPγ-NAT1-Clox | MK652108 | loxP-NAT1-cre-loxP | GFPγ | S1-XFP | S2 | 5538 |
| pFA-GFPγ-URA3-Clox | MK652109 | loxP-URA3-cre-loxP | GFPγ | S1-XFP | S2 | 5306 |
| pFA-3xGFPγ-LAL | MK652100 | loxP-ARG4-loxP | 3xGFPγ | S1-XFP | S2 | 4576 |
| pFA-3xGFPγ-LHL | MK652101 | loxP-HIS1-loxP | 3xGFPγ | S1-XFP | S2 | 3870 |
| pFA-3xGFPγ-LUL | MK652102 | loxP-URA3-loxP | 3xGFPγ | S1-XFP | S2 | 3976 |
| pFA-3xGFPγ-NAT1-Clox | MK652103 | loxP-NAT1-cre-loxP | 3xGFPγ | S1-XFP | S2 | 6972 |
| pFA-3xGFPγ-URA3-Clox | MK652104 | loxP-URA3-cre-loxP | 3xGFPγ | S1-XFP | S2 | 6740 |
| pFA-mCherry-LAL | MK652115 | loxP-ARG4-loxP | yEmCherry | S1-XFP | S2 | 3139 |
| pFA-mCherry-LHL | MK652116 | loxP-HIS1-loxP | yEmCherry | S1-XFP | S2 | 2433 |
| pFA-mCherry-LUL | MK652117 | loxP-URA3-loxP | yEmCherry | S1-XFP | S2 | 2539 |
| pFA-mCherry-NAT1-Clox | MK652118 | loxP-NAT1-cre-loxP | yEmCherry | S1-XFP | S2 | 5535 |
| pFA-mCherry-URA3-Clox | MK652119 | loxP-URA3-cre-loxP | yEmCherry | S1-XFP | S2 | 5303 |
| pFA-HA-LAL | MK652120 | loxP-ARG4-loxP | 3xHA | S1-HA | S2 | 2518 |
| pFA-HA-LHL | MK652121 | loxP-HIS1-loxP | 3xHA | S1-HA | S2 | 1812 |
| pFA-HA-LUL | MK652122 | loxP-URA3-loxP | 3xHA | S1-HA | S2 | 1918 |
| pFA-HA-NAT1-Clox | MK652123 | loxP-NAT1-cre-loxP | 3xHA | S1-HA | S2 | 4914 |
| pFA-HA-URA3-Clox | MK652124 | loxP-URA3-cre-loxP | 3xHA | S1-HA | S2 | 4656 |
| pFA-myc-LAL | MK652125 | loxP-ARG4-loxP | 5xmyc | S1 | S2 | 2614 |
| pFA-myc-LHL | MK652126 | loxP-HIS1-loxP | 5xmyc | S1 | S2 | 1908 |
| pFA-myc-LUL | MK652127 | loxP-URA3-loxP | 5xmyc | S1 | S2 | 2014 |
| pFA-myc-NAT1-Clox | MK652128 | loxP-NAT1-cre-loxP | 5xmyc | S1 | S2 | 5010 |
| pFA-myc-URA3-Clox | MK652129 | loxP-URA3-cre-loxP | 5xmyc | S1 | S2 | 4798 |
| pFA-TAP-LAL | MK652130 | loxP-ARG4-loxP | TAP | S1 | S2 | 2986 |
| pFA-TAP-LHL | MK652131 | loxP-HIS1-loxP | TAP | S1 | S2 | 2280 |
| pFA-TAP-LUL | MK652132 | loxP-URA3-loxP | TAP | S1 | S2 | 2386 |
| pFA-TAP-NAT1-Clox | MK652133 | loxP-NAT1-cre-loxP | TAP | S1 | S2 | 5382 |
| pDIS-NAT1-Clox | MK652134 | loxP-NAT1-cre-loxP | - | N5-up | N5-dw | 5448 |
| pDIS-URA3-Clox | MK652135 | loxP-URA3-cre-loxP | - | N5-up | N5-dw | 5216 |