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. 2019 Apr 17;13(4):421–429. doi: 10.5009/gnl18408

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Copyright © 2019 by The Korean Society of Gastroenterology, the Korean Society of Gastrointestinal Endoscopy, the Korean Society of Neurogastroenterology and Motility, Korean College of Helicobacter and Upper Gastrointestinal Research, Korean Association the Study of Intestinal Diseases, the Korean Association for the Study of the Liver, Korean Pancreatobiliary Association, and Korean Society of Gastrointestinal Cancer.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — siN-BLRs suppressed cell migration and invasion. (A) Wound healing assay was observed by microscopy at 0 and 24 hours. Scale bar refers to 100 μm. (B) Matrigel invasion assay was performed using an invasion chamber after knockdown of N-BLR expression. After treatment with siRNAs, AGS and MKN28 cells were stained with 0.1% coomassie brilliant blue. Scale bar refers to 100 μm. (C) EMT markers were detected by immunoblotting in transfected AGS and MKN28 cells. (D) The influence of transient transfection with siN-BLR on the miR-200 family. MiR-200c-3p was increased in both N-BLR small interfering RNA (siRNA)-transfected cell lines compared with cells transfect-ed with the scramble control. The data shown in the figures are from three independent experiments and represent the mean±SEM. *p<0.05 and ^†p<0.01 show a statistically significant difference compared with a scrambled control.

EMT, epithelial-to-mesenchymal transition.

Fig. 3 — siN-BLRs suppressed cell migration and invasion. (A) Wound healing assay was observed by microscopy at 0 and 24 hours. Scale bar refers to 100 μm. (B) Matrigel invasion assay was performed using an invasion chamber after knockdown of N-BLR expression. After treatment with siRNAs, AGS and MKN28 cells were stained with 0.1% coomassie brilliant blue. Scale bar refers to 100 μm. (C) EMT markers were detected by immunoblotting in transfected AGS and MKN28 cells. (D) The influence of transient transfection with siN-BLR on the miR-200 family. MiR-200c-3p was increased in both N-BLR small interfering RNA (siRNA)-transfected cell lines compared with cells transfect-ed with the scramble control. The data shown in the figures are from three independent experiments and represent the mean±SEM. *p<0.05 and ^†p<0.01 show a statistically significant difference compared with a scrambled control.

EMT, epithelial-to-mesenchymal transition.