TeNT did not affect lysosome fusion. A, Synapto-pHluorin signal in control and TeNT-treated cultured astrocytes (control, n = 14 cells; TeNT, n = 15 cells). White outline marks an astrocyte according to epifluorescence microscopy. Scale bars, 10 μm. B, EGFP-LAMP1 signals in control and TeNT-treated cultured astrocytes (control, n = 14 cells; TeNT, n = 12 cells). Scale bars, 10 μm. C, Statistics of number of mechanical simulation-induced fusion events marked by EGFP-LAMP1 in control and TeNT-treated astrocytes. All events occurring within 10 s after the stimulus were counted. D, Statistics of average imaged area in control and TeNT-treated astrocytes. n.s., p > 0.05; Student's t test. Bars depict mean ± SEM.