Figure 6.

Effects of HSP70 overexpression on the expression of Aβ-degrading enzymes. A, B, Total RNA was extracted from the brains of 12-month-old APPsw/WT (n = 10) and APPsw/HSP70 mice (n = 8) (A) or from primary cultures of neurons, astrocytes and microglia prepared from WT/WT (n = 3) and WT/HSP70 mice (n = 3) (B). RNA samples were subjected to real-time RT-PCR using a specific primer for each gene. Values were normalized to gapdh gene expression and expressed relative to the control sample. Values are given as mean ± SEM (A) or SD (B). **p < 0.01; *p < 0.05; n.s., not significant.