Skip to main content
. 2011 Apr 6;31(14):5512–5525. doi: 10.1523/JNEUROSCI.5548-10.2011

Figure 4.

Figure 4.

Chronic DA treatment changes larval motor behavior. A, B, Representative Western blot (A) and densitometric analyses (B) of extracts prepared from brains of 5 dpf larvae incubated with MPTP for 2 d. The decrease of TH expression is MPTP concentration dependent. C, Representative confocal images confirm the decrease of TH levels in the brain of 5 dpf larvae treated with MPTP. Samples were immunostained with TH (red) and counterstained with Hoechst (blue). There is a decrease of TH labeling in the Pr of MPTP-treated larvae (ii, iv, vi, viii) when compared with control (i, iii, v, vii). D, E, Number of movements initiated during 30 min after drug washing, by 5 dpf larvae drug treated for 2 d. D, The depression in the motor behavior of MPTP-treated larvae is concentration dependent. E, MPTP, DA, and Qui treatment suppresses movement episodes. Larvae treated with DA+MPTP result in increased movement episodes. Pr, Pretectum; TeO, optic tectum; DT, dorsal thalamus; VT, ventral thalamus; PT, posterior tuberculum; Hr, rostral hypothalamus; Ctrl, control. Scale bar, 50 μm. N = 3–4 larvae per group; data are average ± SD. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 compared with control. ###p ≤ 0.001 compared with DA+MPTP.