DA treatment for 2 d downregulates GSK3β activity. A–F, Representative Western blot (A, C, E,) and densitometric (B, D, F) analyses of extracts prepared from brains of 5 dpf larvae DA-treated for 2 d. A, B, There is no change in the levels of pAkt(Thr308) in the 5 dpf larvae treated with DA over 2 d. C, D, DA does not modulate pGSK3β(Ser9) after 30 min of treatment. E, F, The level of GSK3β(Ser9) is reduced in the brain of 5 dpf larvae treated with DA for 2 d. Ctrl, Control. N = 3–4 larva per group; data are average ± SD. *p ≤ 0.05.