Figure 5.

VAMP3 and VAMP7 silencing in primary oligodendrocytes. OPCs were siRNA transfected and differentiated for 3–4 d to allow endogenous PLP expression. A, Surface-localized and total PLP were determined by immunostaining using O10 and aa3 antibodies, respectively. Scale bars, 50 μm. B, Odyssey infrared scan depicting total PLP and surface PLP of control siRNA-transfected and VAMP3/VAMP7-cosilenced oligodendrocytes. A detail showing an identical field of the scan is shown. C, Surface PLP normalized to total PLP calculated from Odyssey scans of siRNA-treated cells (n = 5 for single and n = 4 for double silencing experiments). Error bars depict SEM. Paired t test, *p < 0.05, **p < 0.01.