Basic membrane and firing properties of juvenile DNLL neurons. A, APs were induced by 1 ms current injections ranging from 0 to 1500 pA. The inset shows a magnified view of the APs. B, Persistent firing was observed during 300 ms current injections. From the smallest hyperpolarizing current steps, the membrane resistance and membrane time constant were estimated. Depolarizing current steps from 0 to 700 pA were used to estimate the maximal firing frequency (F) and the IO-F (G). C, Membrane time constant as a function of resting potential. D, Membrane time constant as a function of membrane resistance Rinput. E, AP half-widths were determined from the first suprathreshold current injection as shown in A and are plotted as a function of Rinput. F, Maximal firing frequency extracted from the IO-F and plotted as a function of the AP voltage threshold. The voltage threshold was derived from APs elicited by the first suprathreshold current injections. G, IO-Fs at different recording temperatures.