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. 2011 Mar 23;31(12):4555–4568. doi: 10.1523/JNEUROSCI.6582-10.2011

Figure 1.

Figure 1.

CLIP-170 is needed for proper dendritic arbor morphology of hippocampal neurons. A, Representative images of hippocampal neurons cotransfected at DIV7 for 5 d with control pSuper vector or CLIP-170sh#1, #2, #3, or #4, stained with antibody against endogenous CLIP-170 and β-galactosidase for visualization of morphology of transected cells. Scale bar, 10 μm. B, Efficiency of shRNAs was estimated by analysis of average intensity of CLIP-170 immunostaining from transfected cells. Error bars indicate SEM. C, COS7 cells were cotransfected for 2 d with shRNA constructs targeting rat CLIP-170 (sh#3 and #4) or empty vector, together with plasmids encoding GFP-CLIP-170, and then immunoblotted for the presence of exogenous protein. D, Representative images of neurons in hippocampal slice cultures transfected with pSuper vector or CLIP-170sh#1 together with GFP, fixed 4 d after transfection. Scale bar, 100 μm. E, Number of apical and basal dendritic tips of hippocampal neurons in slice cultures after transfection with four different shRNAs (#1–4) against CLIP-170. Error bars indicate SEM (***p < 0.001, **p < 0.01, *p < 0.05; Kruskal–Wallis test).