Figure 3.
Effects of CLIP-170 depletion on the development of dendritic arbor. Hippocampal neurons at DIV6 were transfected with CLIP-170sh#3TRE or pSuperTRE and β-actin-GFP. Doxycycline was added at DIV7 and neurons were fixed immediately or at DIV9. A, Schematic representation of a time course of the experiment and representative images of transfected neurons. Scale bar, 20 μm. B–E, TNDT (B), TDL (C), Sholl analysis (D), and CDL (E) of hippocampal neurons after CLIP-170 knockdown. Error bars indicate SEM (***p < 0.001, *p < 0.05; Kruskal–Wallis test).