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. 2011 Mar 30;31(13):5120–5130. doi: 10.1523/JNEUROSCI.4807-10.2011

Figure 5.

Figure 5.

RhoA is required to maintain neural stem/progenitor cells. A, Immunohistochemistry for the stem/progenitor marker Sox2 and neuronal marker NeuN. B, Immunohistochemistry for Doublecortin (DCX) showed a qualitative increase of early neurons in RhoA mutant spinal cords. C, D, Quantification of staining showed in B. The percentage of NeuN-positive cells is increased in the mutants (C), and consequently the percentage of Sox2-positive cells is decreased (D). The percentage of NeuN-positive cells increases from 27.81 ± 0.39% in the control to 39.74 ± 0.75% at E10.5 (n = 3, p = 0.0001) and from 63.11 ± 0.84 to 69.87 ± 0.65% at E11.5 (n = 3, p = 0.0035). Meanwhile, the Sox2-positive fraction decreases from 72.19 ± 0.39% in the control to 60.26 ± 0.75% in the mutant at E10.5 (n = 3, p = 0.0001) and from 36.92 ± 0.85% in the control to 30.14 ± 0.65% in the mutant at E11.5 (n = 3, p = 0.0031). Scale bars, 100 μm.