Aβ25–35 increases the Et uptake in microglia of acute hippocampal slices. A–D, Representative images of acute hippocampal brain slices showing microglia positive for IB4 (green) and Et uptake (red) under control conditions (A, B) or treated for 3 h with 10 μm Aβ25–35 (C, D). E, Averaged data normalized to control (dashed line) of Et uptake rate of hippocampal microglia treated with Aβ25–35 (10 μm; 3 h) or treated with Aβ25–35 plus the following blockers applied during dye uptake recording: Gap26 (200 μm), Cx43E2, Prob (500 μm), or 10panx1 (200 μm). Averaged data were obtained from three independent experiments. *p < 0.005, effect of 10 μm Aβ25–35 for 3 h compared with control; #p < 0.05, ###p < 0.001, effect of each blocker compared with the respective effect induced by 10 μm Aβ25–35 for 3 h. Scale bar, 20 μm. Error bars indicate SEM.