Figure 12.
Blockade of connexin and pannexin hemichannels reduces neuronal death induced by Aβ25–35 in acute hippocampal slices. A–C, Representative images of Et uptake (red) and F-Jade-positive staining (green) by pyramidal neurons under control conditions (A) or treated for Aβ25–35 (10 μm; 3 h) alone (B) or plus 200 μm La3+ (C). D, Averaged number of F-Jade-positive cells per field of pyramidal neurons under control conditions or treated for 3 h with Aβ25–35 (10 μm). Shown is the effect of La3+ (200 μm), Gap26 (200 μm), Prob (500 μm), 10panx1 (200 μm), or minocycline (Mino) (10 μm). Also shown is the neuronal death in hippocampal astrocytes of Cx43fl/fl: GFAP-cre (Cx43−/−) mice. ***p < 0.001, **p < 0.005, *p < 0.05, compared with control. ###p < 0.001, ##p < 0.005, #p < 0.05, respective blocker effect compared with the Aβ (10 μm; 3 h) effect. Averaged data obtained from five independent experiments. Scale bar, 80 μm. Error bars indicate SEM.