Figure 8.
Cell proliferation is not affected by Aβ25–35 and hemichannel blockers. A, B, Representative immunofluorescence images depicting isolectin GS-IB4 (green) and CD-11b (red) immunolabeling in microglial cultures under control conditions (A) or after 72 h treatment with 10 μm Aβ25–35 (B). C, Quantification of the total number per field of CD-11B positive microglia under control conditions or treated by 72 h with Aβ25–35 (10 μm). *p < 0.05; compared with control. Averaged data obtained from four independent experiments. Scale bar, 20 μm. D, Proliferation of microglia (gray bars) and astrocytes (black bars) untreated (0 h of treatment) or treated for several time periods with 10 μm Aβ25–35. E, Proliferation of microglia (gray bars) and astrocytes (black bars) treated for 72 h with 10 μm Aβ25–35. In some experiments, 200 μm Gap26, Cx43E2, 200 μm 10panx1, 500 μm Prob were coapplied with Aβ25–35. Error bars indicate SEM.