Figure 9.

Aβ_25–35 reduces cell viability only at long period of exposure. A, Percentage comparing total cells of terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL)-positive microglia (gray bars) or astrocytes (black bars) untreated (0 h of treatment) or treated for several time periods with 10 μm Aβ_25–35. B, Percentage comparing total cells of TUNEL-positive microglia (gray bars) or astrocytes (black bars) treated for 72 h with 10 μm Aβ_25–35. In some experiments, 200 μm Gap26, 200 μm Gap27, Cx43^E2, 200 μm ¹⁰panx1, 500 μm Prob were coapplied with Aβ_25–35. C and D are similar experiments to A and B, respectively, showing neuronal death evaluated with F-Jade. Averaged data were obtained from three independent experiments. *p < 0.05; compared with control. Error bars indicate SEM.