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. 2011 Nov 2;31(44):15660–15673. doi: 10.1523/JNEUROSCI.2592-11.2011

Figure 2.

Figure 2.

Analysis of pros and miR-279 mutants reveals specific and general effects on ORN number and targeting. A, MP ORNs were quantified using the general OR marker Or83b-GFP (Or83b-GAL4, UAS-mCD8GFP). The number of ORNs in prosIG2227 clones was increased compared with control clones. This increase correlated with the number of ectopic CO2 neurons in the mutants. The number of ORNs in pros17 clones was decreased by ∼10 ORNs on average compared with control clones. B, eyFLP mosaic analysis was used to analyze ORN projection patterns in the AL of several classes of ORNs. miR-279 and pros mutants show very mild mistargeting in few classes of non-CO2 receptor ORNs. In general, mistargeting was mild and random. Pros mutants display a reduction of Or22a and Or47a neurons, as shown by the reduced innervations of the AL glomerulus. C, Ectopic CO2 neurons on the MP coexpress Or42a and Or59c. Examples of whole-mount in situ hybridization on control and mutant MP clones expressing the Gr21a-mCD8GFP reporter are shown. Scale bars: B, 50 μm; C, 10 μm.