Figure 2.

Phenotype of nestin-deficient mice. A, Nestin-deficient mice showed reduced falling time compared to their wild-type littermates in the Roto-Rod test (n = 5 for WT and n = 11 for Nes^−/−, *p < 0.05, Student's t test). B, No significant difference was found in muscle strength of Nes^−/− mice compared with wild-type littermates (n = 5 for WT and n = 11 for Nes^−/−). C, Nes^−/− mice had more AChR clusters compared to wild types. AChR clusters in the Nes^−/− mice are dispersed in a larger muscle territory. Quantitation of this is shown in D (n = 5 for WT and n = 5 for Nes^−/−) and E (n = 6 for WT and n = 6 for Nes^−/−, 3 measurements taken for each sample; data are represented as mean ± SEM, *p < 0.05 and **p < 0.01, Student's t test).