Increased AChR cluster bandwidth in nestin knock-out mice is accompanied by extended projections of motor axons. A, Diaphragms of E16.5 Nes−/− and their wild-type littermates were stained with α-bungarotoxin, neurofilament 150 and 200, synaptophysin, and SV2 antibodies. B, Higher-magnification view of AChR clusters. C, Axon length is increased in nestin knock-out samples (n = 3 for WT and n = 3 for Nes−/−, data are represented as mean ± SEM, **p < 0.01, Student's t test).