Table 1.
Expression patterns of fluorescent proteins in the glomerular layer in transgenic mouse lines used in this study
| Markers |
VGAT-Venus |
TH-Cre;Rosa26R-CFP |
TH-GFP |
|||
|---|---|---|---|---|---|---|
| Marker+ [%] | Venus+ [%] | Marker+ [%] | CFP+ [%] | Marker+ [%] | GFP+ [%] | |
| TH | 20.0 ± 0.3 | 98.1 ± 0.7 | 72.1 ± 1.5 | 64.5 ± 1.1 | 82.7 ± 1.4 | 98.1 ± 0.6 |
| (n = 2120) | (n = 432) | (n = 1571) | (n = 1762) | (n = 804) | (n = 676) | |
| CB | 15.0 ± 0.3 | 98.9 ± 0.2 | 0 ± 0 | 0 ± 0 | 0.27 ± 0.1 | 0.57 ± 0.3 |
| (n = 2274) | (n = 344) | (n = 713) | (n = 404) | (n = 760) | (n = 350) | |
| CR | 34.5 ± 3.2 | 79.5 ± 3.9 | 0.13 ± 0.1 | 0.05 ± 0.1 | 0 ± 0 | 0 ± 0 |
| (n = 1142) | (n = 495) | (n = 763) | (n = 1943) | (n = 368) | (n = 934) | |
| GAD67 | 99.4 ± 0.2 | 99.2 ± 0.2 | 98.5 ± 0.03 | 19.0 ± 0.2 | 95.1 ± 0.7 | 22.9 ± 1.2 |
| (n = 1166) | (n = 1169) | (n = 783) | (n = 4055) | (n = 362) | (n = 1508) | |
The table shows the percentage of fluorescently-labeled PGCs that expressed TH, CB, CR, or the GABAergic neuron marker, GAD67 (Marker+ [%]) and the percentage of PGCs expressing each neuronal marker that also expressed a fluorescent protein (Venus+, CFP+, or GFP+ [%]). Both TH−/CFP + cells in TH-Cre;Rosa26R-CFP and TH−/GFP + cells in TH-GFP mice express Pax6 (100 ± 0% and 95.7 ± 0.8%, respectively), which is an early marker for DAergic PGC differentiation (Hack et al., 2005; Kohwi et al., 2005; Haba et al., 2009), but not CB or CR, suggesting that they are immature DAergic PGCs. “n” indicates the number of cells (from three animals) analyzed here.