Figure 2.

Differentiation of wt and ogg1^−/− NSCs. A, Growth factor removal-induced differentiation of NSCs is manifested as reduced levels of nestin and corresponding elevations in the neuronal Tuj-1 and astrocytic GFAP markers. B, Progressive reduction of neurogenesis and increased astrogenesis from ogg1^−/− NSCs. Left, Differentiating cells from wt and ogg1^−/− NSCs are morphological similar (B-W, black and white phase-contrast images). Neurons and astrocytes at differentiation day 1 and 5 were identified by immunocytochemistry using antibodies against Tuj-1 (middle) and GFAP (right). C, Quantitative analysis of neurons and astrocytes at day 1 and day 5 in differentiation. Gene expression analyses, presented relative to wt at day 1. Data are mean with SD from more than three independent experiments. *p < 0.05; **p < 0.01.