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. 2011 Jun 29;31(26):9445–9455. doi: 10.1523/JNEUROSCI.0011-11.2011

Figure 3.

Figure 3.

BDNF signaling through the Ras–ERK cascade is not altered in dendrites of Ts1Cje hippocampal neurons. The fluorescence intensity of the indicated immunolabeled protein was quantified in the dendrites of WT and Ts1Cje hippocampal neurons, in the presence or absence of BDNF (100 ng/ml for 10 min). A, Significant increases in phospho-Mnk1 (Thr197/202) were observed in BDNF-treated versus untreated wild-type cells (**p = 0.008, t test) and in BDNF-treated versus untreated Ts1Cje cells (**p = 0.010, t test). No significant difference was detected between nontreated Ts1Cje and wild-type neurons (p = 0.618, t test); n = 6. B, Significant increases in phospho-eIF4E (Ser209) were observed in BDNF-treated versus untreated wild-type cells (*p = 0.014, t test) and in BDNF-treated versus untreated Ts1Cje cells (**p = 0.005, t test). No significant difference was detected between untreated Ts1Cje and wild-type neurons (p = 0.992, t test); n = 4. Data are expressed as the mean ± SEM. Representative immunocytochemistry images are shown in each case, and MAP2 was used as a dendritic marker. Scale bars, 60 μm.