Figure 6.

Rapamycin restores mTOR activity in hippocampal Ts1Cje neurons. A, B, Phospho-mTOR (Ser2448) fluorescence intensity was quantified in dendrites of WT and Ts1Cje hippocampal neurons at DIV12 in the presence or absence of rapamycin (rapa, 20 nm) for 10 min (A) or 24 h (B), as indicated. Rapamycin produced a significant decrease in the Ts1Cje neurons (10 min: ***p < 0.001; 24 h: **p = 0.002, t test), while only a slight decrease was observed in wild-type cells treated with rapamycin for 24 h (*p = 0.022, Mann–Whitney test). As expected, Ts1Cje neurons exhibited stronger dendritic labeling than wild-type neurons in basal conditions (10 min: **p = 0.002, t test; 24 h: ***p < 0.001, Mann–Whitney test). Data are expressed as the mean ± SEM (n = 7–11). Representative immunocytochemistry images are shown in each case, and MAP2 was used as a dendritic marker. Scale bars, 60 μm.