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. 2011 Jun 29;31(26):9445–9455. doi: 10.1523/JNEUROSCI.0011-11.2011

Figure 8.

Figure 8.

The NMDAR inhibitors APV, memantine, and MK-801 decrease mTOR activity in hippocampal Ts1Cje neurons. A, Phospho-mTOR (Ser2448) fluorescence intensity was quantified in dendrites of WT and Ts1Cje hippocampal neurons at DIV12 in control conditions or in the presence of APV (120 μm), MK-801 (10 μm), or memantine (30 μm) for 10 min or 2 h, as indicated. The three compounds significantly decreased mTOR (Ser2448) phosphorylation in Ts1Cje neurons both at 10 min (***p < 0.001, Mann–Whitney test for APV and MK-801, t test for memantine) and 2 h (***p < 0.001, t test for APV and memantine, Mann–Whitney test for MK-801). As expected, Ts1Cje neurons exhibited stronger dendritic labeling than wild-type neurons in basal conditions (***p < 0.001, t test). Data are expressed as the mean ± SEM (n = 9–10). B, Representative immunocytochemistry images of experiments summarized in A (10 min treatments) are shown; MAP2 was used as a dendritic marker. Scale bars, 60 μm.