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. 2011 Aug 17;31(33):11814–11824. doi: 10.1523/JNEUROSCI.6804-10.2011

Figure 4.

Figure 4.

IGF signaling regulates the timing of GnRH2 neuron emergence but not their spatial locations. A, Blockage of IGF signaling delays the timing of emergence of GnRH2 neurons. Tg(hsp70:dnigf1r-GFP) embryos were treated as described in Figure 2A. GnRH2 neurons were detected by whole-mount in situ hybridization at the indicated time points. Ventral views are shown with anterior up. Arrows indicate GnRH2 neurons. The location of eyes are indicated by e. B, C, Blockage of IGF signaling in advanced embryos decreases GnRH2 neuron migration rate. Tg(hsp70:dnigf1r-GFP) embryos were treated with or without two 0.5 h HS treatments at 32 and 44 hpf. GnRH2 neurons were detected by whole-mount in situ hybridization the indicated time points. Ventral views are shown with anterior up. Arrows point to the positions of GnRH2 neurons (B). The migration index, defined as the length between right and left GnRH2 neuronal clusters divided by the length between the centers of the retinas, was quantified and shown in C. Values are shown as mean ± SE. n = 15–19, *p < 0.01 compared with the no (w/o) HS group of the same developmental age.