Figure 9.

CanA-14F, CanB, and CanB2 genes are expressed ubiquitously in the adult brain. In situ hybridization and immunohistochemistry were performed as described in Materials and Methods. A, C, E, Representative frontal sections of the male fly head showing the brain, optic lobes, and retina labeled with DIG-labeled antisense RNA probes for CanA-14F (A), CanB (C), and CanB2 (E). B, D, F, Sense RNA probes were used as control. All tissue sections were counterstained with Nuclear Fast Red following in situ hybridization. The box areas are shown in higher magnification in A′–F′. Whole-mount adult male brains of control (elav-Gal4;UAS-Dicer-2 × w¹¹¹⁸) (G) and CnB#2 RNAi (elav-Gal4;UAS-Dicer-2 × CnB#2) (H) were stained with an anti-Calcineurin B antibody. Representative maximum projection images of 1.5 μm confocal sections of the entire brain are shown. Scale bars, 100 μm.