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. 2011 Sep 14;31(37):13118–13127. doi: 10.1523/JNEUROSCI.2331-11.2011

Figure 2.

Figure 2.

Colocalization of Vsx1 and the GUS8.4GFP reporter in putative Type 7 ON cone bipolar cells. Vsx1 immunolabeling (A) in adult retinal sections from mice harboring the GUS8.4GFP reporter transgene is detected in putative Type 7 ON bipolar cells coimmunolabeled with GFP (B, arrowhead). The image in C shows the merged images of A and B. Putative rod bipolar cells exhibiting low levels of GFP immunolabeling (AC, arrow) do not colabel with Vsx1. In Vsx1τLacZLacZ mice, β-galactosidase (D) and GFP (E) coimmunolabeling is observed in putative Type 7 bipolar cell (DF, arrows). Some β-galactosidase-expressing cells (DF, white arrowhead) do not colabel with GFP, while some weakly immunolabeled GFP cells do not express the Vsx1:τ-β-galactosidase reporter (DF, open arrowheads). The dashed lines in AC indicate the boundary of the inner nuclear layer. Scale bars: AC, 30 μm; DF, 15 μm.