Colocalization of Vsx1 and the GUS8.4GFP reporter in putative Type 7 ON cone bipolar cells. Vsx1 immunolabeling (A) in adult retinal sections from mice harboring the GUS8.4GFP reporter transgene is detected in putative Type 7 ON bipolar cells coimmunolabeled with GFP (B, arrowhead). The image in C shows the merged images of A and B. Putative rod bipolar cells exhibiting low levels of GFP immunolabeling (A–C, arrow) do not colabel with Vsx1. In Vsx1τLacZ/τLacZ mice, β-galactosidase (D) and GFP (E) coimmunolabeling is observed in putative Type 7 bipolar cell (D–F, arrows). Some β-galactosidase-expressing cells (D–F, white arrowhead) do not colabel with GFP, while some weakly immunolabeled GFP cells do not express the Vsx1:τ-β-galactosidase reporter (D–F, open arrowheads). The dashed lines in A–C indicate the boundary of the inner nuclear layer. Scale bars: A–C, 30 μm; D–F, 15 μm.