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. 2011 Sep 21;31(38):13485–13499. doi: 10.1523/JNEUROSCI.2136-11.2011

Figure 6.

Figure 6.

PTPδ is required for induction of presynaptic differentiation by IL1RAPL1. A–C, Immunostaining for Bassoon of cocultures of cortical neurons derived from Ptprd+/+, Ptprd+/−, or Ptprd−/− mice and HEK293T cells expressing IL1RAPL1 (A), FLAG-NLGN1 (B), or N-cadherin (C). D, Intensity of staining signals for Bassoon on the surface of HEK293T cells expressing IL1RAPL1, FLAG-NLGN1, and N-cadherin (n = 21–24). E, Immunostaining for Synapsin1 of cocultures of cortical neurons derived from Ptprd+/+ or Ptprd−/− mice and HEK293T cells expressing HA-IL1RAPL1 or HA-NGL-3. F, Intensities of staining signals for Synapsin1 on the surface of HEK293T cells expressing HA-IL1RAPL1 and HA-NGL-3 (n = 30–32). G, Expression levels of HA-IL1RAPL1 and HA-NGL-3 on the surface of HEK293T cells. HEK293T cells transfected with HA-IL1RAPL1 or HA-NGL-3 were immunostained with anti-HA under nonpermeabilized conditions. Intensities of fluorescent staining signals were quantified (n = 32 and 35 for HA-IL1RAPL1 and HA-NGL-3, respectively). All values represent mean ± SEM. **p < 0.01, ***p < 0.001, Tukey's test in D and F, and Student's t test in G. Scale bars, 10 μm.