Table 1.
Sequence of primers used in real-time PCR, amplicon sizes, and their melting temperatures
| Target genes | Sequences | Amplicon length | Tm (°C) |
|---|---|---|---|
| Slc12a2 (NKCC1) | F: AAGGGCAGCGAGGAAGCCAA | 135 | 61 |
| R: GCCGTTCTGGAAGCTCACGTT | |||
| Slc12a4 (KCC1) | F: AACGAGGTCATTGTCACGCGCT | 147 | 62 |
| R: ACGCACCAACAACACCCGTT | |||
| Slc12a5 (KCC2) | F: TGCCATCAAACGGCTCAGGTGT | 68 | 62 |
| R: TTGAGCCAAGCAACAGGGCAGT | |||
| Slc12a6 (KCC3) | F: AGTGAAGATGCTCGCGCTTGGA | 170 | 62 |
| R: AGCATGCCCCCATCATGCACAA | |||
| Slc12a7 (KCC4) | F: AACTGGCGTCCACGCTTCAAGT | 122 | 62 |
| R: CCGGCAATGAGCATGGCGAAAA | |||
| Il6 | F: ACAACCACGGCCTTCCCTACTT | 100 | 62 |
| R: AGCCTCCGACTTGTGAAGTGGT | |||
| Il6ra | F: TGTTGAAGACTCTGCCAACCACGA | 145 | 62 |
| R: ACAGAGAAGCAACCCAAACGCCAA | |||
| R: TGGTCAAATCTTTGCGCAGCCC | |||
| Polr2j | F: ACCACACTCTGGGGAACATC | 160 | 59 |
| R: CTCGCTGATGAGGTCTGTGA |
Slc12a, solute carrier 12 family; Il6, interleukin 6; Il6ra, interleukin 6 receptor α chain; Polr2j, polymerase (RNA) II polypeptide J; corresponding protein is in parentheses. F, Forward; R, reverse.