Table 1.

Experimental procedures

Experiment (Figure)	Treatment	Cell labeling	Perfusion	Maze training	Maze probe
Anterograde tracer (1a–d)	Unilateral EC tracer infusion		7 d after infusion
S-induced Fos (1e–h)	Unilateral EC S		1 h after S
S-induced DG proliferation (2), apoptosis (4f–h)	Unilateral EC S, NS	BrdU 1, 3, 5, or 7 d after S	24 h after BrdU
S-induced DG survival (3d,e)	Unilateral EC S, NS	BrdU 1, 10, or 30 d before S	3 weeks after S
S-induced SVZ proliferation (4a–c)	Unilateral EC S, NS	BrdU 3 d after S	24 h after BrdU
Cerebellar S (4d,e)	Unilateral cerebellar S, NS	BrdU 3 d after S	24 h after BrdU
Retroviral labeling of DGCs (5)	Unilateral EC S, NS	Bilateral DG retrovirus infusion 3 d after S	1 or 6 weeks after infusion
S-induced DG neurogenesis (3a–c) and DGC integration (6)	Unilateral EC S, NS	IdU 3–5 d, CldU 7–9 d after S	∼10 weeks after XdU	Standard training ∼1 or 6 weeks after XdU	∼10 weeks after S
S effects on spatial memory (7, 8)	Bilateral EC S, NS			Undertraining 1.5 or 6.5 weeks after, or 1 d before S	1 h after training or 1.5 weeks after S
TMZ/S effects on spatial memory (9)	TMZ or V before bilateral EC S, NS	BrdU 3–5 d or ∼7 weeks after S	∼7 weeks after S	Undertraining 6.5 weeks after S	1 h after training

NS, Nonstimulation; S, stimulation.