Skip to main content
. 2011 Jun 8;31(23):8476–8490. doi: 10.1523/JNEUROSCI.1598-11.2011

Figure 2.

Figure 2.

Two distinct subpopulations of neurons expressing VGluT2 mRNA in the A10 region (−5.28 mm from bregma). A, Low magnification of a 5-μm-thick coronal section with TH immunoreactivity in the A9 and A10 regions. B–C′ correspond to the same section at two different magnifications showing TH immunoreactivity under bright-field microscopy (B, C) or VGluT2 mRNA under epiluminescence microscopy (B′, C′) in the different divisions of the A10 region. In C and C′, the boxes delimit areas shown at higher magnification of the lateral parabrachial pigmental area [PBP(l)] in D and D′, medial PBP [PBP(m)] in E and E′, the RLi in F and F′, and the IF in G and G′. D–G′, TH-IR neurons coexpressing VGluT2 mRNA (thin arrows), examples of neurons expressing VGluT2 mRNA and lacking TH immunoreactivity (thick arrows), and examples of TH-IR neurons with background levels of silver grains (arrowheads). fr, Fasciculus retroflexus. Scale bar: (in G′) A, 393 μm; B, B′, 238 μm; C, C′, 145 μm; D–G′, 50 μm.