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. 2011 Jun 8;31(23):8476–8490. doi: 10.1523/JNEUROSCI.1598-11.2011

Figure 9.

Figure 9.

Two subpopulations of neurons expressing VGluT2 mRNA innervate the nAcc. A–D, Low magnification of coronal sections (18 μm thick) from a rat injected with FG into the nAcc. TH immunofluorescent in cell bodies and fibers (A, C) or FG-labeled cells (B, D). Boxed areas in A–D are shown in A′–D′, respectively, in which arrows indicate FG-labeled cells shown at higher magnification in 1A–6A (TH immunofluorescent), 1B–6B (FG fluorescent), 1C–6C (FG detection with an anti-FG antibody), 1D–6D (VGluT2 mRNA seen as aggregates of green grains), and 1E–6E (VGluT2 mRNA seen as aggregates of white grains). 1A–1E, A FG-labeled cell containing TH immunoreactivity and lacking VGluT2 mRNA (arrowheads). 2A–2E, A FG-labeled cell lacking TH immunoreactivity but containing VGluT2 mRNA (long arrows). 3A–3E, A FG-labeled cell lacking both TH and VGluT2 mRNA (double-headed arrows). 4A–4E, A FG-labeled cell with TH immunoreactivity and VGluT2 mRNA (short arrows). 5A–6E, FG-labeled cells lacking TH immunoreactivity but containing VGluT2 mRNA (long arrows). Scale bar: (in 6E) A–D, 258 μm; A′–D′, 80 μm; 1A–6E, 40 μm.