Table 4.

Frequency of neurons expressing VGluT2 mRNA containing or lacking TH immunoreactivity in the CLi

	Percentage of VGluT2-only neurons or VGluT2-TH neurons within the total population of VGluT2 mRNA-expressing neurons		Percentage of TH-only neurons or VGluT2-TH neurons within the total population of TH-IR neurons
	VGluT2-only neurons	VGluT2-TH neurons	TH-only neurons	VGluT2-TH neurons
Bregma (in mm)
−6.24 to −6.36
Subject 1	70.8% (n = 17)	29.2% (n = 7)	85.7% (n = 42)	14.3% (n = 7)
Subject 2	77.8% (n = 35)	22.2% (n = 10)	81.1% (n = 43)	18.9% (n = 10)
Subject 3	79.5% (n = 51)	20.5% (n = 14)	82.7% (n = 64)	17.3% (n = 14)
Mean ± SEM	76.9 ± 2.9% (n = 103)	23.1 ± 2.9% (n = 31)	83.0 ± 2.6% (n = 149)	17.0 ± 2.6% (n = 31)
−6.48
Subject 1	74.5% (n = 41)	25.5% (n = 14)	82.5% (n = 66)	17.5% (n = 14)
Subject 2	90.0% (n = 36)	10.0% (n = 4)	89.5% (n = 34)	10.5% (n = 4)
Subject 3	82.1% (n = 32)	18.0% (n = 7)	86.3% (n = 44)	13.7% (n = 7)
Mean ± SEM	82.2 ± 4.5% (n = 109)	17.8 ± 4.5% (n = 25)	86.0 ± 2.0% (n = 144)	14.0 ± 2.0% (n = 25)
−6.60 to −6.72
Subject 1	100% (n = 13)	0% (n = 0)	100% (n = 56)	0% (n = 0)
Subject 2	86.7% (n = 65)	13.3% (n = 10)	91.0% (n = 101)	9.0% (n = 10)
Subject 3	80.8% (n = 38)	19.2% (n = 9)	86.6% (n = 58)	13.4% (n = 9)
Mean ± SEM	88.5 ± 4.0% (n = 116)	11.5 ± 4.0% (n = 19)	92.2 ± 3.3% (n = 215)	7.8 ± 3.3% (n = 19)

VGluT2-only neurons (expressing VGluT2 mRNA but lacking TH immunoreactivity), VGluT2-TH neurons (coexpressing VGluT2 mRNA and TH immunoreactivity), or TH-only neurons (containing TH immunoreactivity but lacking VGluT2 mRNA) were counted in every 16th 5-μm-thick coronal section from three different rats (subjects 1–3). Cell counting was done at three different levels of the CLi: −6.24 to −6.36 mm from bregma (a total of 3 sections from 3 rats), −6.48 mm from bregma (a total of 3 sections from 3 rats), and −6.60 to −6.72 mm from bregma (a total of 4 sections from 3 rats). n, Counted neurons.